Journal: bioRxiv

Article Title: Mitochondria-derived dsRNA-induced stress granules promote IRF3-mediated fibrotic responses

doi: 10.1101/2025.05.14.654103

Figure Lengend Snippet: (A-C,F,G) LX2 cells were transfected with scrambled control siRNA or siRNA against IRF3 ( A-C,G ) or IFNAR ( F ) and then stimulated with 5 ng/mL TGFβ for 24 hours. (A,F,G) Expression of indicated mRNAs was assessed by qRT-PCR. C T values were normalized to 18S rRNA and are shown as negative Δ C T for a more intuitive data representation. (B) Western blot was performed on conditioned media to assess Type I collagen protein expression with quantification in ( C ). (D-E) LX2 cells were stimulated with 5 ng/mL TGFβ for 16 hours, then immunofluorescence was performed with anti-IRF3 antibody and DAPI to indicate nuclei. 3D reconstructions of Z-stacks were created with IMARIS, and the Surfaces module was used to depict nuclei. (D) Representative top-down views showing DAPI (blue, top row), total IRF3 (green, top row), nuclei (blue, bottom row), or IRF3 inside nuclei (pink, bottom row). Quantification of IRF3 fluorescence inside the nucleus is shown in ( E ). Data was normalized to the nuclear volume of the field and are depicted as fold change relative to unstimulated cells. For all graphs, each dot represents the mean of an individual experiment, and the error bars indicate the cumulative mean ± SEM. Values with different alphabetical superscripts are significantly different, P < 0.05.

Article Snippet: The next day, cells were transfected with scambled control siRNA or a pool of three siRNAs against human G3BP2 (encodes G3BP2 protein) (sc-89231, Santa Cruz, Dallas, TX).

Techniques: Transfection, Control, Expressing, Quantitative RT-PCR, Western Blot, Immunofluorescence, Fluorescence

Journal: bioRxiv

Article Title: Mitochondria-derived dsRNA-induced stress granules promote IRF3-mediated fibrotic responses

doi: 10.1101/2025.05.14.654103

Figure Lengend Snippet: LX2 cells were ( A-C ) pre-treated for one hour with 25 μM EGCG or vehicle or ( D-F ) transfected with scrambled control siRNA or siRNAs against G3BP1 and G3BP2 and then stimulated with 5 ng/mL TGFβ for 24 hours. (A,D) Expression of COL1A1 and FN1 mRNA was analyzed by qRT-PCR. Values were normalized to 18S rRNA and are shown as negative Δ C T for a more intuitive data representation. ( B,E) Western blot was performed on conditioned media to assess Type I collagen and fibronectin protein expression with quantification in ( C ) and ( F ), respectively. For all graphs, each dot represents the mean of an individual experiment and the error bars indicate the cumulative mean ± SEM. Values with different alphabetical superscripts are significantly different, P < 0.05.

Article Snippet: The next day, cells were transfected with scambled control siRNA or a pool of three siRNAs against human G3BP2 (encodes G3BP2 protein) (sc-89231, Santa Cruz, Dallas, TX).

Techniques: Transfection, Control, Expressing, Quantitative RT-PCR, Western Blot

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 3. Time course of toluene concentrations in assays conducted with C. vulgaris, R. opacus or a consortium of these microorganisms with nitrate or a combination of ammonium and nitrate as nitrogen sources. Initial concentra tion of toluene was 3 mg L−1 in all the conditions.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques:

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 4. Time course of (A) phenol, (B) benzene, (C) toluene, (D) ethylbenzene and (E) o-xylene concentrations in C. vulgaris-R. opacus co-cultures under an air atmosphere.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques:

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 5. Time course of phenol and BTEX concentrations in (A) Mix 1, (B) Mix 2 and (C) Mix 3 in C. vulgaris-R. opacus cultures cultivated with in a mixture of the five pollutants under air atmosphere. Initial concentrations of the pollutants (100 %) in the mixture were 25 mg L−1 of phenol, and 3, 3, 8 mg L−1 for benzene and toluene, 1, 3, 3 mg L−1 for ethylbenzene; and 0.5, 1, 1 mg L−1 for o-xylene in Mix 1, 2 and 3, respectively.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques:

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 6. Time course of (A) phenol, (B) benzene, (C) toluene, (D) ethylbenzene and (E) o-xylene concentration in C. vulgaris-R. opacus cultures under a N2/CO2 (70/30 %) atmosphere.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques: Concentration Assay

Journal: Journal of Water Process Engineering

Article Title: Developing a microalgal-bacterial consortium for the removal of organic pollutants from petrochemical industry

doi: 10.1016/j.jwpe.2025.107663

Figure Lengend Snippet: Fig. 7. Time course of phenol and BTEX concentration in (A) Mix 1, (B) Mix 2 and (C) Mix 3 in C. vulgaris-R. opacus cultures incubated with a mixture of the five pollutants under a N2/CO2 (70/30 %) atmosphere. Initial concentrations of the pollutants (100 %) in the mixture were 25 mg L−1 of phenol, and 3, 3, 8 mg L−1 for benzene and toluene, 1, 3, 3 mg L−1 for ethylbenzene; and 0.5, 1, 1 mg L−1 for o-xylene in Mix 1, 2 and 3, respectively.

Article Snippet: The microorganisms used in this work were the freshwater microalga Chlorella vulgaris SAG 211-11b (SAG Culture Collection of Algae, Germany) and the bacterium Rhodococcus opacus DSM 43205 (DSMZ, Germany).

Techniques: Concentration Assay, Incubation